Abstract:Objective：To explore the effect of hepatic intra-arterial injection of emulsions of lipidol and pingyangmycin (EL+P) on normal hepatic tissues.
Methods ：Forteen big-ear white rabbits were divided into three groups: sham-operation group, A group (low dose of EL+P),and B group(high dose of EL+P). Laparotomy was performed, and various doses of EL+P were perfused into the hepatic artery. Biopsy specimens were obtained on the 1st, 2nd, 4th, and 6th week respectively after the operation. Hematoxylin-eosin stain, immunohistochemistry stain of platelet derived growth factor B(PDGF-B) and quantitative analysis were performed.
Results：The main manifestation of A group was transient hepatocyte hydropic degeneration; in group B the degeneration of hepatocytes was most severe in the 2nd week. Fibrosis appeared in the 4th week,and obvious pseudolobules were formed in some parts of the liver in the 6th week. The expression of PDGF-B was mainly concentrated on the membrane of hepatocytes and fibrotic septum.
Conclusions：Hepatic intra-arterial injection of EL+P can result in damage of normal hepatic tissue. PDGF-B participated in the process of hepatic fibrosis.