Abstract:Objective：To investigate how to prevent injury to non-heart-beating donor (NHBD) in rat orthotopic liver transplantation and improve the success rate of operation.
Methods：Male SD rats were divided into non-heart-beating time 30min (N-30) and non-heart-beating time 45min (N-45) group randomly.Thirty liver transplants were performed in each group. Meanwhile,a routine operation group and a modified operation group were also set up according to the surgical procedure of donor operation.
Results：(1) In routine operation and modified operation group cold ischemia time was (70.04±1.48) and (70.36±1.42)min(P>0.05) respectively,but anhepatic phase, IVC clamping time and recipient operation time in both groups was (70.32±1.53)min, (16.40±0.73)min,(22.75±1.16)min and(90.58±3.76)min, respectively; (2) In N-30 and N-45 groups, there were 5 and 9 recipients who died of primary graft non-function(PGN) in normal group,but only 1 and 2 recipients, respectively, died of PGN in modified operation group(40% Vs 12%,P<0.05).(3)In N-30 and N-45 groups, 5 and 7,2 and 2,2 and 1 recipients,respectively, died of grafted liver bleeding after reperfusion ,deep anesthesia, and prolongation of anhepatic phase. (4) One-week survival rate was 50% and 30% in N-30 and N-45 group respectively(P<0.05).
Conclusions：The key to improve the success rate of rat orthotopic liver transplantation is to prevent these factors,including injury in donor removal, grafted liver bleeding after reperfusion,deep anesthesia, and prolongation of anhepatic phase.