Abstract:Objective：To evaluate the effect of perioperative enteral immunotrition(IMPACT) on liver regeneration in cirrhotic rats with hepatectomy.
Methods ：Forty-eight cirrhotic rats were randomly divided into two groups: Group A, perioperative standard enteral nutrition group(n=24), receiving standard enteral nutrient NUTRISON during hepatectomy. Group B, perioperative enteral immunonutrition group(n=24), receiving enteral immunonutrient IMPACT after hepatectomy. According to the different time of taking specimens, each group was separated into four subgroups, each subgroup had six rats. The rats in the two groups received equal daily nutritional supplement intragastrically, which was 690kJ/kg per day. Before the 68% hepatectomy, the rats were fed with enteral nutrient for 8 days, and after operation were fed until the day of taking specimens. On the day before hepatectomy and 1st, 4th and 8th postoperative day(POD), MI (mitotic index) of liver cell and PCNA (proliferating cell nuclear antigen) labeling index of hepatocyte were determined.
Results：MI increased significantly in both group on 4th and 8th postoperative day(P<0.05), with the maximal value on 4th day after hepatectomy. The values of MI in B3 and B4 subgroups were significantly higher than those in A3 and A4 subgroups(P<0.05). PCNA labeling index increased significantly in both groups on 1st, 4th and 8th day(P<0.05), with the maximal value on 1st day after hepatectomy. The values of PCNA labeling index in B3 and B4 subgroups were significantly higher than those in A3 and A4 subgroups(P<0.05).
Conclusions：Compared with perioperative standard enteral nutrition, perioperative enteral immunonutrition can enhance the regeneration capacity of the remnant liver and maintain a high ability of regeneration for a longer time after resection of cirrhotic liver of rats.