Abstract:Objective:To study the significance of change of high mobility group box 1 (HMGB1) level of pancreas in acute necrotizing pancreatitis (ANP) in rats.
Methods :ANP model was induced by retrograde injection of sodium taurocholate in pancreatic duct. Animals were divided randomly into three groups: control group, ANP group, and sodium butyrate treatment group (treatment group). The serum levels of TNF-α and IL-1β were measured by ELISA. The HMGB1 mRNA level of pancreas was detected by RT-PCR.
Results:The serum levels of TNF-α and IL-1β were quickly increased after the model was induced, and reached a peak at 6h, but decreased at 12h. The HMGB1 mRNA level of pancreas was increased significantly at 12h, and maintained to 24h. Whereas in treatment group, the HMGB1 level of pancreas was lower than ANP group (P<0.05) and injury of pancreas was milder than that of ANP group (P<0.05).
Conclusions:HMGB1 seems to act as a late mediator in ANP. Sodium butyrate treatment could decrease HMGB1 levels of ANP rats.