Abstract:Objective:To investigate the levels of C peptide, insulin, glucagon and glucose and the function of islet endocrine cells in rats with acute pancreatitis. Methods:one hundred and tweenty rats were randomly divided to two groups: Group A (acute pancreatitis,AP) and Group B (sham operation,SO). Blood C peptide, insulin, glucagon and glucose were determined. The pancreatic morphology was observed after laparotomy. Pancreas tissue was excised to be observed in terms of histopathology.
Results:The levels of C peptide, insulin and glucagon in group A were significantly higher than those in the group B at 9h, 15h and 12h after the procedure respectively (P<0.01,P<0.05 and P<0.05, respectively). Histopathologjcally, the rats in group A had severe acute pancreatitis at 9h after the procedure. Conclusions:(1)The islet endocrine cells function of rats with acute pancreatitis is impaired, the levels of C-peptide and insulin in serum can reflect the function of islet B cells. C-peptide can more accurately and more quickly reflect the degree of B cells injury, and its changes are synchronous with the histopathological changes in B cells. (2)The level of glucagon can reflect the function of pancreas A cells. The islet A cells may be more susceptible than B cells to be damaged in acute pancreatitis.