Abstract:Objective:To explore the effects of heat shock protein 27 (HSP27) expression on adhesion junction of cells during severe acute pancreatitis (SAP) and mild acute pancreatitis (MAP) in rats.
Methods:Ninty male Wistar rats were divided randomly into three groups: Group A (SAP), Group B (MAP) and group C (control) . Rats in the three groups were killed at 1h, 3h.and 8h after model made respectively. The levels of serum amylase (SAM) and histopathology score were measured. Changes of adhesion junctions were examined by electron microscopy. HSP27 and its phosphorylation were measured by Western-blotting. The expression of HSP27 mRNA was determined by RT-PCR.
Results:Levels of SAM increased in group A and group B, but was markedly lower in Group B (P<0.05) . In group A, there was a rapid and sustained increase in levels of pancreatic indexes, and severe interstitial edema (P<0.05), and damages of adhesion junctions were observed. In group B, there was moderate pancreatic edema and disruption of adhesion junctions. The expression of HSP27, its mRNA and its phosphorylation levels were lower in group A than those in group B（P<0.05）.
Conclusions:Alterations of adhesion junction are a common feature of AP, but the damage of HSP27 and its phosphorylation which regulate adhesion junctions were more severe during SAP. The results suggest that HSP plays an important role in the onset and development of AP.