Abstract:Objective:To study the difference of expression of proteins in pancreatic cancer and adjacent fissue.
Methods :Proteomes of eight pairs of pancreatic ductal adenocarcinoma tissue samples and adjacent tissue samples were obtained by high resolution two-dimensional gel electrophoresis (2-DE). Comprehensive analyses of proteins were focused on total protein spots exhibiting statistical alternations between the two groups. Protein identification was done by peptide mass fingerprinting with tandem mass spectrometry （MS/MS）. In addition, Western blotting and immunohistochemistry were performed to verify the expression of certain candidate protein.
Results:A total of 28 protein spot-features were found to be significantly increased and 17 significantly decreased in tumor tissues. Thirty of these protein spots were identified, which included enzymes, antioxidant proteins, signal transduction proteins, calcium-binding protein, structural proteins, chaperones and others. Western blotting and IHC further validated up-regulated expressions of one candidate protein (annexin II) in tumor tissues.
Conclusions:The analysis of proteomics with 2-DE on human tissue is a useful method for discovering valuable cancer marker candidates. These differential expressed proteins may serve as biomarkers for early detection and therapeutic targets to pancreatic cancer.