Abstract:Objective:To study the cotransfection mB7-1 and mCD1D gene into pancreatic cancer cells of rats and to observe its anti-tmor responses.
Methods :Recombinant retroviral vectors expressing mB7-1and mCD1D gene were packaged into GP2-293 cell lines and transfected. The expressions of mB7-1 and mCD1D were detected with PCR and Western blot. The positive cells of mB7-1 and mCD1D were used to induce the anti-tumor immunity in vitro.
Results: Anti-tumor immunity was induced after B7-1 and CD1D positive cells were coinoculated in syngeneic mice. Furthermore, the growth of tumor was inhibited.
Conclusions:Cotransfection of B7-1 and CD1D could induce anti-tumor effect. This study provide a foundation for the application of B7-1 and CD1D gene therapy in tumor.