Abstract:Objective:To study the effect of antisense mTOR gene transfection mediated by nanoparticles(NP) on intimal perliferation after vein grafting.
Methods :Nanoparticle antisense mTOR gene complex was prepared with PLGA and PVA. Autogenous vein graft model was established in 72 rats by transplanting internal branch of jugular vein to carotid artery. Three groups were studied: (1) antisense mTOR group, antisense mTOR gene mediated by NP was transfected into the veins before anastomosis. (2) Empty vector group, the vein was transfected by empty vector mediated by NP. (3) Control group, no transfection. The grafted veins were harvested 3 days, 1 week, 2 weeks and 4 weeks after operation, respectively. The exogenous mTOR mRNA and protein expression were determined and intimal hyperplasia (IH) was observed. The presence of apoptotic VSMC was also detected.
Results:Antisense mTOR gene transfection mediated by nanoparticle complex inhibited the mRNA and protein expression of mTOR gene(P<0.05), and the IH was inhibited in the vein graft especially during 7d~28d(P<0.01).
Conclusions:Nanoparticle is an effective gene transfecting carrier, and antisense mTOR gene expression can prevent the IH and promote apoptosis after vein grafting.