Abstract:Objective:To investigate whether trichostatin A (TSA) possesses antitumor activity against human colon cancer cells.
Methods :Human colon cancer cell line Lovo was treated with different concentrations of TSA. The growth of Lovo cells were observed by MTT assay before and after TSA treatment. The cell cycle of Lovo cells was analyzed by flow cytometry.
Results:Trichostatin A significantly inhibited the proliferation of colon cancer cells at 500 ng/ml. The inhibition rate increased sharply from day 2 to day 5 (57.21% to 82.76%). 20 ng/ml TSA treatment could induce cell cycle arrest at the G1 phase, but with no apparent increase in apoptotic cells. ≥100 ng/ml TSA could induce significant apoptosis as shown by flow cytometry.
Conclusions:The results indicate that TSA is able to inhibit colon cancer cell growth in vitro, possibly through G1 cell cycle arrest and induction of apoptosis. This study suggests that TSA may be a potential therapeutic agent for the target treatment of colon cancer.