Abstract:Objective To investigate the expression of T lymphoma invasion and metastasis inducing factor 1(Tiam 1) in gastric cancer cells and analyse the correlation between Tiam 1 and the invasive and migratory potential of gastric cancer cells in vitro and in vivo.
Methods Two subpopulations lesser(ML) and higher(MH) adhesive subgroup were separated from human gastric cancer cell line MKN45(M0) by laminin adhesion method in vitro. RTPCR and ELISA were applied to detect the expression of Tiam 1 mRNA and protein in M0, ML and MH cells. The invasive and migratory potential of M0, ML and MH cells in vitro and in vivo were observed by Boyden chamber and by inoculation into nude mice and, simultaneously, correlation between the expression of Tiam 1 and the invasive and migratory potential of gastric cancer cells was analysed.
Results The invasive and migratory potential of MH cells in vitro (24.33±8.02, 52.00±14.53) and the lung metastatic rate of MH cells in nudemice (4/5 = 80%) was much higher than that of M0 cells (11.67±3.79, 26.00±9.54, 2/5 = 40%, RV = 0.759±0.047, RD = 0.911±0.104) and ML cells (9.67±3.06, 23.67±8.50, 1/5 = 20%, RV = 0.743±0.039, RD = 0.892±0.101) (P< 0.05), 〖JP3〗as well as the expression of Tiam 1 mRNA (RV = 0.855±0.051) and protein (RD = 1.262±0.165) in MH cells were much higher than those in MO and ML cells (P< 0.05),