Abstract:Objective To establish a method for amplification of immature dendritic cells(DC) from murine bone marrow in vitro and investigate correlations between maturation degree of DC and varying dosages of granulocytemacrophagecolony stimulating factor (GMCSF).
Methods Dendritic cells from murine bone marrow were cultured with different dosages of rm GMCSF. The suspension cells were examined with scanning electronic microscope,and the nonsensitized T lymphocyte proliferation was observed by mixed lymphocyte reaction.
Results DC cultured in lower dosage of rmGMCSF (GMlow DC) exhibited typical characteristics of DC, and had immature characteristics in cell phenotype and cell functions with high expression of CD11c and low expression of CD80,CD86 and MHC II on the surface of the cells.The ability of GmlowDC to stimulate the proliferation of nonsensitized T lymphocyte in vitro was weaker than that of GmhighDC.
Conclusions The methods of immature DCs culturing establised by allthors was feasible. The dosage of rm GMCSF has a direct relationship with the maturation degree of DC.