Abstract:Objective:To investigate the cytotoxicity of ADM-PBCA-NP on L-02 cells.
Methods : L-02 cells were cultured in vitro and the LDH activity of supernatant liquid of culture cells was examined. The toxicity of ADM-PBCA-NP, ADM and PBCA-NP to L-02 cells by the MTT assay was also determined, and the haemolysis function of PBCA-NP with different concentrations was detected.
Results:The cytotoxicity of ADM-PBCA-NP, ADM and blank PBCA nanoparticles to L-02 cells under the 10-6 mol/L concentration range was not cytototic (grade 1). LDH activity of supernatant liquid of culture cells showed no differences between the 3 groups.
Conclusions:Nanoparticles of ADM-NP and PBCA-NP in the 10-6 mol/L concentration range have no significant toxic effect on L-02 hepatic cells; and in a certain concentration range, the cell compatibility is excellent and will not lead to hemolysis.