Abstract:Objective:To observe the effect of PKB gene silencing on the growth of gastric cancer cell line SGC-7901 in vitro.
Methods :Gene transfection technique was used to transfect AKt2 siRNA into gastric cancer cells. Akt2 expression was detected by RNAi technique, Akt2 protein level was detected by Western blot, and the change of cell cycle distribution and apoptosis of SGC-7901 cells were detected by flow-cytometry, SGC-7901 proliferation was measured by MTT method.
Results:After SGC-7901 cells transfected with Akt2 siRNA, the expression of protein level decreased obviously (P＜0.01）. Compared with control group, the transfected group had more SGC-7901 cells accumulated at G2/M phase, and proliferation rate was reduced (P＜0.01）. It also showed that the transfected group had apoptosis ladder, and the cell growth concentrated at G2/M phase.
Conclusions:siRNA targeting Akt2 could inhibit the proliferation of SGC-7901 cells in vitro. Induction of apoptosis and inhibition of PI3/K signaling pathway may be involved in its mechanism. Akt2 could be a novel target for gene therapy of gastric cancer.