Abstract:Objective:To determine the changes of gene expression profile in small intestinal allograft in rats after ischemic preconditioning (IPC), and to study the mechanism of graft protection of IPC.
Methods :Rats are randomly divided into 3 groups: sham operation (S group), small bowel transplant (SBT group) and IPC small bowel transplant group (ISBT group). Total RNAs was extracted from intestine of the 3 groups 1h after the intestine remored and cold preservation/reperfusion, and then purified to mRNA. mRNAs was then reversely transcribed to cDNA and to prepare hybridization probes. The mixed probes were hybridized to the cDNA microarray.After high-stringent washing, the fluorescent signals on cDNA microarray were scanned and analyzed.
Results:Among the 4 096 target genes, 297 differentially expressed genes were identified between normal intestine and intestinal allograft in ISBT group; among those 84 genes which have been reported, including 18 genes expressing down and 66 genes expressing up regulation. Differentially expressing genes could be related to the protective effect of IPC.
Conclusions:The mechanisms of protective effect of IPC on cells of the graft are by modulation of genes related to cell adhesion, related to cellular energy and metabolism, and related to the signal transmission of the cells.