Background and Aims There has been no consistent conclusion about the effect of Glissonean pedicle transection method and Pringle's maneuver in hepatectomy for hepatocellular carcinoma (HCC), and has no verification from the large multicenter clinical trials as well at present time. This study was conducted to evaluate the efficacy of the two methods in hepatectomy for HCC through Meta-analysis, so as to provide reference for the selection of inflow occlusion methods during hepatectomy for HCC.Methods The clinical studies comparing Glissonean pedicle transection method and Pringle's maneuver in liver resection for HCC were collected by computer-based searching in Chinese and English databases, with the retrieval time from inception of the database to October 2021. After the quality assessment and data extraction of the included studies, Meta-analysis was performed with RevMan5.3 software.Results A total of 15 studies was included involving 1 349 patients, of whom, Glissonean pedicle transection was used in 684 cases (Glissonean pedicle transection group) and Pringle's maneuver was performed in 665 cases (Pringle group). Results of Meta-analysis showed that the levels of alanine aminotransferase (MD=-96.67, 95% CI=-156.69--36.65, P=0.002; MD=-55.57, 95% CI=-87.03--24.11, P=0.000 5; MD=-41.33, 95% CI=-70.92--11.73, P=0.006) and aspartate aminotransferase (MD=-71.92，95% CI=-130.02--13.81, P=0.02; MD=-41.45, 95% CI=-60.62--22.28，P=0.000 1; MD=-38.89, 95% CI=-71.14--6.65， P=0.02) on postoperative day (POD) 1, 3 and 7 as well as the levels of total bilirubin (MD=-4.47, 95% CI=-7.75--1.18, P=0.002; MD=-5.09, 95% CI=-10.05--0.12, P=0.04) on POD 3 and 7 were lower in Glissonean pedicle transection group than those in Pringle group, and there were no significant differences in albumin levels on POD 1, 3 and 7 between the two groups (all P>0.05); the amount of intraoperative blood loss (MD=-98.48, 95% CI=-145.53--51.43, P<0.000 1) and transfusion rate (OR=0.25, 95% CI=0.13--0.46, P<0.000 1) in Glissonean pedicle transection group were lower than those in Pringle group, and the operative time had no significant difference between the two groups (P>0.05); the overall postoperative complications (OR=0.31, 95% CI=0.22--0.43, P<0.000 1) and hospital stay (MD=-2.94, 95% CI=-4.12--1.76, P<0.000 01) were less than those in Pringle group.Conclusion Glissonean pedicle transection is a safe and effective vascular occlusion method in hepatectomy for HCC. Compared with Pringle's maneuver, Glissonean pedicle transection can effectively alleviate liver injury, reduce intraoperative bleeding, postoperative complications and shorten hospital stay. However, surgeons should select a suitable method for occlusion based on their own personal experience, patients' conditions, and specific situation during surgery.