Background and Aims Long non-coding RNA PCAT19 (lncRNA PCAT19, hereafter referred to as PCAT19) is upregulated in various tumors and closely associated with malignant progression and poor prognosis. However, no studies have reported the expression, function, and mechanisms of PCAT19 in pancreatic cancer. The authors predicted that PCAT19 could interact with miR-195-5p in a complementary manner through the starBase database. Therefore, this study was conducted to investigate the expression and function of PCAT19 in pancreatic cancer cells, as well as its regulatory relationship with target genes and corresponding miRNA.Methods The expressions of PCAT19 and miR-195-5p in human pancreatic ductal epithelial cells (HPNE) and pancreatic cancer cell lines (PANC-1, SW1990, HS766T, CFPAC-1) were detected by qRT-PCR method. Dual-luciferase reporter gene analysis was conducted to assess the targeted binding interaction between PCAT19 and miR-195-5p. PANC-1 cells were transfected with PCAT19 silencing sequence si-PCAT19 (si-PCAT19 group), negative control sequence (si-NC group), and miR-195-5p inhibitor plus si-PCAT19 (co-transfection group). Then, cell proliferation ability was determined using MTT assay, cell invasion ability was assessed using Transwell assay, and the expressions of proteins related to the Wnt/β-Catenin signaling pathway were determined by Western blot analysis.Results In all studied pancreatic cancer cell lines, the expression levels of PCAT19 were significantly higher than that in HPNE cells. In contrast, the expression levels of miR-195-5p were significantly lower than that in HPNE cells (all P<0.05). Dual-luciferase experiments showed that miR-195-5p was a target miRNA of PCAT19. In the si-PCAT19 group compared with the si-NC group, the expression of miR-195-5p was significantly increased, cell proliferation and invasion abilities were significantly decreased, and the expression levels of β-catenin, c-Myc, and cyclin D1 were significantly downregulated (all P<0.05). However, in the co-transfection group, there were no statistically significant differences in these indexes compared with the si-NC group (all P>0.05).Conclusion PCAT19 is upregulated in pancreatic cancer cells and can promote the proliferation and invasion of pancreatic cancer cells. The mechanism may be related to regulating miR-195-5p and affecting the Wnt/β-Catenin signaling pathway.