Abstract:
Objective:
To investigate the effect of recombinant human endostatin (rhES) on the growth of transplanted gastric cancer in nude mices. Methods :At the 20 day after tumor transplantation, twelve Balb/c-nu/nu mice were randomly divided into two groups:(1)Control group: 0.9% NS 0.1ml was injected around the tumor,once a day for 10 days; (2)experimental group: rhES 2mg/kg was inpected around the tumor once a day for 10 days. The tumors bulk were measured every five days until five days after injection. VEGF, bFGF,PDGF,VEGF-c, VEGFR-3， FⅧAg, PCNA, bcl-2 and apoptosis index were examined. The tumor inhibition rate(TIR) and tumor condensing rate (TCR) were calculated. The difference of AI , tumor bulk and MVD between the 2 groups were assessed. Results :The difference of the tumor bulk between the two group was no statistical significance before injection, but at the time of 15 days after begining of the injection, the difference was significant(P =0.0001); and in the experimental group the tumor bulk was also decreased significantly after treatment(P=0.0015). MVD, VEGF, bFGF, PDGF， VEGF-c，VEGFR-3 and bcl-2 expressed lower in experimental group than those in control group; PCNA expression was the same in the 2 groups. AI increased significant in experimental group compared with in control group (P =0.0000). In experimental group,the TIR was 91.2%,TCR 53.5%,and HE staining presented more necrotic areas.Conclusions :rhES can inhibit angiogenesis of the tumor and promote tumor cell apoptosis, so that can inhibit the growth of tumor.
