Objective: To investigate the safety and feasibility of the nipple complex (NAC) sparing modified mastectomy with first-stage breast reconstruction in treatment of early breast cancer. Methods: During 2010 to 2014, 170 breast cancer patients, according to their own choice, received NAC sparing modified mastectomy with first-stage breast reconstruction (observational group, 102 cases), or modified subcutaneous mastectomy with NAC removal (control group, 68 cases), respectively. The relevant postoperative parameters and postoperative patient’s satisfaction with breast reconstruction and quality of life as well as the incidence of adverse events, recurrence and metastasis during follow-up of the two groups were compared. Results: The duration and volume of wound drainage showed no significant difference between the two groups (both P<0.05); the postoperative patient satisfaction rate (97.06% vs. 51.47%), overall FACT score (150.89±25.34) vs. (100.24±18.47) and high satisfaction rate (83.33% vs. 51.47%) in observational group were significantly higher than those in control group (all P<0.05); there was no significant difference in incidence of adverse events, local recurrence and distant metastasis between the two groups (all P>0.05). Conclusion: NAC sparing modified mastectomy with first-stage breast reconstruction is safe and feasible for early breast cancer, and it is evidently superior to modified subcutaneous mastectomy without NAC preservation with regard to postoperative cosmetic results and improving patient’s quality of life.

Objective: To compare the efficacy of ultrasonic scalpel and traditional electrocautery for mastectomy and axillary lymph node dissection in breast cancer surgery.
Methods: The relevant randomized controlled trials (RTCs) were collected by searching the national and international databases. After literature screening, quality assessment and data extraction, Meta-analysis was performed by using RevMan 5.2 software. 
Results: Eight RTCs were finally included, with a total sample size of 604 cases comprising 300 cases in ultrasonic scalpel group and 304 cases in electrocautery group. Results of Meta-analysis showed that in ultrasonic scalpel group compared with electrocautery group, the operative time had no significant difference (WMD=6.20, 95% CI=-5.02–17.41, P=0.28), but the intraoperative blood loss (WMD=–61.42, 95% CI=–83.76––39.09), postoperative drainage volume (WMD=–374.92, 95% CI=–453.90––295.95), drainage time (WMD=–3.30,
95% CI=–5.10––1.49), and the incidence of subcutaneous fluid collection (RR=0.51, 95% CI=0.38–0.68) were all significantly decreased (all P<0.05).
Conclusion: In breast cancer surgery, use of ultrasonic scalpel is superior to that of electrocautery in respects of intraoperative bleeding control, and reducing postoperative drainage and incidence of subcutaneous fluid collection.

Objective: To investigate the Skp2 and p27 Kip1 expressions in breast cancer tissue and the significance.
Methods: The Skp2 and p27Kip1 expressions in normal breast tissues, atypical ductal hyperplasia (ADH) tissue and invasive ductal carcinoma (IDC) tissue were determined by immunohistochemical staining. The relations of their expressions with lymph node metastasis and histologic grade of breast cancer were also analyzed.
Results: The Skp2 positive expression rate was increased in ascending order in normal breast tissue, ADH tissue and IDC tissue, which was significantly higher in IDC tissue with axillary lymph node metastasis than that in IDC tissue without axillary lymph node metastasis, while the opposite was true for p27Kip1 positive expression rate (all P<0.05). Both Skp2 and p27Kip1 positive expression rates were significantly different among IDC tissues with different histologic grades (both P<0.05), moreover, Skp2 positive expression had a positive correlation with histologic grade of IDC (r=0.492, P<0.05), while p27Kip1 positive expression had a negative correlation with histologic grade of IDC (r=–0.327, P<0.05).
Conclusion: Skp2 expression is up-regulated and p27Kip1 expression is down-regulated in breast cancer tissue, and their changing degrees are closely related to the malignant biological behaviors of breast cancer.

Objective: To investigate the expression of collagen triple helix repeat containing 1 (CTHRC1) in pancreatic cancer tissue, and its influence on biobehavior of pancreatic cancer cells.
Methods: The expression of CTHRC1 mRNA in the specimens of cancer and adjacent tissue from 40 pancreatic cancer patients was detected by real time PCR (RT-PCR). Pancreatic cancer Panc28 cells were transfected with pcDNA3.1-CTHRC1 or CTHRC1 siRNA using untransfected Panc28 cells as control, and then the cell proliferation, migration and invasion ability were determined by colony formation assay, wound healing assay and Boyden chamber assay, respectively.
Results: Results of the RT-PCR showed that CTHRC1 expression level in pancreatic cancer tissue was significantly higher than that in adjacent tissue (P<0.05). Compared with control Panc28 cells, the number of colony formation was increased, percentage of wound width was reduced and number of invaded cells was increased significantly in Panc28 cells with up-regulated CTHRC1 expression after pcDNA3.1-CTHRC1 transfection, while the opposite changes were seen in those with down-regulated CTHRC1 expression after CTHRC1 siRNA transfection, and all the differences had statistical significance (all P<0.05).
Conclusion: CTHRC1 expression is increased in pancreatic cancer tissue, which may contribute to the occurrence and development of pancreatic cancer through promoting cell proliferation, and migration and invasion ability of the pancreatic cancer cells.

Objective: To compare the effects of ultrasound-guided Mammotome rotation cutting and wire-localized surgical resection for non-palpable breast lesion (NPBL), so as to provide the basis for procedure selection in clinical practice. Methods: The clinical data of 362 patients with NPBL undergoing surgical treatment were retrospectively analyzed. Of the patients, 293 cases underwent ultrasound-guided Mammotome procedure (rotation cutting group) and the other 69 cases underwent ultrasound-guided wire-localized surgical resection (open group). The relevant surgical parameters between the two groups were compared. Results: The general data between the two groups of patients showed no significant difference (all P>0.05). Rotation cutting group was superior to open group in variables of operative time, length of incision, and intraoperative blood loss, but the surgical cost in rotation cutting group was significantly higher than that in open group (all P<0.05). The incidence of postoperative complications in rotation cutting group was lower than that in open group, but the difference was not significant (P>0.05). The success rate of surgery in both groups reached 100%. No tumor recurrence occurred in the two groups during followed up for 1 month to 12 months, and evident scars of different sizes were seen in open group, while scars were small or invisible in rotation cutting group. Conclusion: Mammotome rotation cutting for NPBL is safe and effective, and has less bleeding, shorter operative time and favorable cosmetic results, but higher cost compared to wire-localized surgical resection.