Objective:To evaluate the transfection characteristic of Dosper and Lipofectamine to establish the basis for future massive application . Methods:The retroviral vector inserted SV40LT antigen gene was transfected into PA317 cell lines by Dosper or Lipofectamine mediation with or without serum. The viral titer was determined with the NIH3T3 cells. Results: In withoutserum condition, the transfection efficiency in Lipofectamine group was higher than that in Dosper group, but no significant difference(P＞0.05); in with serum condition, the transfection efficiency in Dosper group was significant higher than that in Lipofectamine group (P﹤0.01). And the transfection efficiency in both groups with serum were significant lower than that in both without serum groups. Conclusions:Transfection efficiency are high in using Dosper and Lipofectamine in without serum condition.