Objective:To evaluate the relationship between the expression of EGF, EGFR and PCNA with the clinicopathological characteristics in human colorectal carcinoma. Methods :Immunohistochemistry was used to examine the expression of EGF, EGFR and PCNA in 50 specimens of colorectal carcinoma and 10 specimens of normal colorectal tissue. Results:In cancer specimens,the expression of EGF and EGFR were 76.07% and 84.15% respectively, but in normal colorectal tissue were no expression of EGF and EGFR (P<0.05) . The expression of PCNA in tumor tissue was （37.22±14.49）%; and in normal colorectal tissue was （15.12±3.21）% (P<0.05) . The expression of EGF, EGFR and PCNA was not correlated with the size and site of the tumor; however , the expression was significant correlated with the degree of differentiation, Dukes stages, metastasis and recurrence of the colorectal carcinoma. Conclusions :The results suggest that the expression of EGF, EGFR and PCNA is an useful tool for providing information about the malignant degree, prognosis, relapse of the tumor, and may be as a gaiding in postoperative chemotherapy selection for patients with colorectal carcinoma.