ObjectiveTo investigate the effect of nerve growth factor(CNF) on the proliferative ability of human cholangiocarcinoma cell strain QBC939. MethodsAfter construction of a fulllength human βNGF expression vector, human cholangiocarcinoma cell QBC939 was transfected with pcDNA3.0NGF. Western blot assays were used to detect the expression of βNGF. Subsequently. In vitro proliferation before and after transfection of cells was analyzed by MTT assays. ResultsWestern blot assay indicated that βNGF could be stably expressed by transfected QBC939 cells, and compared with controls, the differences were significant (P<0.0001). MTT assays showed stable transfection of βNGF in QBC939 cells resulted in enhanced dosedependent proliferation of cancer cells, and the differences were significant （P<0.01）. ConclusionsNGF may enhance the proliferation of cholangiocarcinoma cells.