ObjectiveTo investigate the influence of selective HGF/cMet inhibitorNK4 against colon cancerand reveal the potential signaling pathway mechanism of NK4 effect on colon cancer cell. MethodsLoVo colon cancer cells were treated with NK4（a selective inhibitor of cMet phosphorylation）at different times. MTT assay and flow cytometry were used to measure cell proliferation and apoptosis. The expression of cMet, pcMet, MEK2, pERK and Cmyc were measured by Western blot. ResultsIn NK4treated group, cells proliferation were inhibited and apoptosis induced in a dose dependent manner, and resulted in significantdownregulation of pcMet and MEK2/ERK pathwayrelated protein. The effect of HGF/on LoVo was the opposite. The ratios of pcMet, MEK2, pERK and Cmyc expression between blank group and the NK4（1μg/mL）treated for 24h group were 2.58, 1.89, 1.67 and 2.21(P<0.01), and for HGF(50mg/mL) group were 0.46, 0.71, 068, 0.58(P<0.01), respectively. ConclusionsThe results showed that selective cMet inhibitor NK4 may inhibit proliferation and induce apoptosis of colon cancer cell lines LoVo through blockade of MEK2/ERK signaling pathway. It may be a new target of selective HGF/cMet inhibitor effect on colon cancer.