To investigate the effect of integrinβ1 antisense oligodeoxynucleotide(ASODN) on humanpancreatic cancinoma transplanted subcutaneously in nude mice.Methods;The models of human pancreaticcancinoma transplanted subcutaneously were established in nude mice, then divided randomly into 3 groups and different treatment was given respectively(control group, random oligodeoxynucleotide group and ASODN group). After treatment, the weight of nude mice and tumor volume were observed, and the tumor growth inhibitory rate and the tumor response rate were calculated. The expressions of integrinβ1 mRNA and protein in tumor tissue were determined by RTPCR and Westernblot. Results;The tumor growth inhibitory rate in the random oligodexynucleotide group and the ASODN group was 4.75％ and 72.70％, respectively. The tumor decrease rate of the ASODN group was 10.91％. The expression level of integrinβ1 mRNA and protein was decreased in the ASODN group compared with other 2 control groups. Conclusions;Our findings suggest that integrinβ1 antisense oligodeoxynucleotides result in marked inhibition of human pancreatic cancinomagrowth in nude mice. It may be a novel treatment approach for human pancreatic carcinoma.