Objective:To investigate the expression and relationship of early growth response gene1(Egr1), plateletderived growth factorB(PDGFB) and tranforming growth factor(TGFβ1) in autogenous vein graft in rats,and the role in vein graft intimal hyperplasia(IH). Methods:Autogenous vein graft model was established in 90 wistar rats. The vein graft samples were harvested at 1，2，6，24 hours, and 3，7，14，28，42 days after surgery. Normal vein was used as control group. Egr1、PDGFB，TGFβ1 mRNA was measured by reverse transcriptionPCR and in situ hybridization. Western blotting and immunohistochemistry were used to detect the protein expression of Egr1，PDGFB and TGFβ1. Results:Expression of Egr1，PDGFB，TGFβ1 mRNA and protein was not detected in normal vein. In grafting vein,expression level of Egr1mRNA reached a peak at 28days, and the positive rate of Egr1mRNA was 45%±6%; PDGFBmRNA reached a peak at 14days（48%±6%）; a peak of TGFβ1mRNA was 46%±9% reached at 7days; Egr1 protein expression reached a peak at 28days, and the positive rate of Egr1 protein was 40%±9%. PDGFB protein reached a peak at 28days（45%±4%）, TGFβ1 protein reached a peak at 14days( 41%±7%). Conclusions:Intimal hyperplasia of vein graft is closely associated withexpression of Egr1、PDGFB and TGFβ1; the activation and expression of PDGFB and TGFβ1 may be modulated by Egr1, and they may contribute to increase expression of Egr1 by feedback.