Abstract:Objective:To analyse the morphological and phenotypic characteristics of dendritic cells obtained by induction of peripheral blood monocytes of gastric cancer patients and provide an experimental basis of dendritic cells for the biological treatment of gastric cancer. Methods:Peripheral blood monocytes of gastric cancer patients were co-cultured with GM-CSF, IL-4 and TNF-α to obtain the dendritic cells. The morphological characteristics of the dendritic cells were observed by light and electron microscopes and the phenotypes were studied by flow cytometry. Results:Mature dendritic cells were oblained seven days after induction culture. These cells showed numerous typical dendritic protuberances on their surface.The phenotypes of the cells increased gradually with their maturation and reached stable levels seven days later. Conclusions:Mature dendritic cells could be obtained from peripheral blood monocytes of gastric cancer patients after induction culture with GM-CSF,IL-4 and TNF-α. The obtained cells had typical morphological and phenotypic characteristics. These cells could be used for further research in the biological treatment of gastric cancer.