Objective：To investigate the feasibility and isolation efficiency of percutaneous selective isolated hepatic perfusion chemotherapy(PSIHP). Methods：Six pigs underwent the procedure of routine transhepatic arterial infusion(HAI) and 6 underwent PSIHP.5FU was used in this study. The drug(5FU) concentration of blood from hepatic and systemic veins of both groups was observed. Liver tissue was investigated for pathologic changes. Results：The peak level of 5FU concentration in blood from right hepatic vein and systemic vein in HAI group was（4082.530±415.213）mg/L and （1682.230±216.834)mg/L respectively.In PSIHP group, the peak level(5FU) was（5321.711±517.318）mg/L and（510.834±52.518）mg/L, respectively.There was a statistically significant difference between HAI group an PSIHP group(P<0.01). The level of 5FU concentration in blood from right hepatic vein of PSIHP group(postfiltration) was（1192.063±114.864）mg/L and the filtration rate of charcoal hemoperfusion was（77.6±0.9%）. The damage of perfused area of liver tissue was more serious in PSIHP group compared with HAI group. Conclusions：PSIHP is a simple and feasible method for isolated hepatic perfusion. Compared with HAI, PSIHP can increase the concentration level of chemotherapeutic agents in regional liver, and can even more markedly decrease the drug concentration in systemic circulation and decrease toxic side effcts.