VEGF-ASODN转染对胃癌细胞VEGF和survivin表达的影响
作者:
通讯作者:
作者单位:

作者简介:

段体德

基金项目:


Influence of VEGF antisense oligonucleotide on expression of VEGF and survivin in gastric cancer cells
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 音频文件
  • |
  • 视频文件
    摘要:

    目的:观察血管内皮细胞生长因子反义寡核苷酸(VEGF-ASODN)转染胃癌细胞SGC-7901对血管内皮生长因子(VEGF)和生存素(survivin)表达的影响。
    方法:人工合成硫代磷酸化VEGF-ASODN。实验分为5组:对照组,错义组,实验1组(转染浓度为100 ng∕mL),实验2组(转染浓度为400 ng∕mL)及实验3组(转染浓度为700 ng∕mL)。转染后实时荧光定量RT-PCR检测细胞RNA起始拷贝数; ELESA法检测细胞及培养上清液VEGF 蛋白含量; Western-blot法检测细胞生存素蛋白表达量; 流式细胞仪检测细胞凋亡; 细胞生长曲线检测转染对细胞生长的影响。
    结果:3个实验组VEGF mRNA水平和VEGF蛋白表达量及培养上清液VEGF 蛋白含量均明显降低,survivin蛋白的表达水平随转染浓度的升高而降低,转染VEGF-ASODN能使胃癌细胞凋亡增加、细胞生长减缓。
    结论:VEGF-ASODN转染胃癌细胞SGC-7901能显著抑制细胞VEGF的表达,促进细胞凋亡,抑制细胞增殖。

    Abstract:

    Abstract:Objective:To observe the effects of VEGF antisense oligonucleotide transfected to SGC-7091 cell line on expression of VEGF and survivin of the cell.
    Methods :The VEGF-ASODN was synthesised artificially. There were five groups in this experiment: control group, missense group and three various concentration groups. After transfection, RNA copies were detected by real-time PT-PCR,VEGF protein in cell and culture supernate was detected by ELESA, survivin protein was measured by Western-blot, and apoptosis was detected by FCM. Growth of cells was evaluated by growth curve.
    Results:VEGF-ASODN tranfection reduced the VEGF mRNA and VEGF protein expression in gastric cancer cells and supernate remarkably. It also reduced survivin protein expression and increased apoptosis in gastric cancer cells.
    Conclusions:Transfection with VEGF-ASODN to gastric cancer cells SGC-7901 can reduce the expression of VEGF, and can increase apoptosis and suppress the proliferation of gastric cancer cells.

    参考文献
    相似文献
    引证文献
引用本文

郑斌, 杨策尧, 段体德. VEGF-ASODN转染对胃癌细胞VEGF和survivin表达的影响[J].中国普通外科杂志,2008,17(10):10-987.
DOI:10.7659/j. issn.1005-6947.2008.10.010

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2008-10-25