Abstract:Objective:To observe the effects of VEGF antisense oligonucleotide transfected to SGC-7091 cell line on expression of VEGF and survivin of the cell.
Methods :The VEGF-ASODN was synthesised artificially. There were five groups in this experiment: control group, missense group and three various concentration groups. After transfection, RNA copies were detected by real-time PT-PCR,VEGF protein in cell and culture supernate was detected by ELESA, survivin protein was measured by Western-blot, and apoptosis was detected by FCM. Growth of cells was evaluated by growth curve.
Results:VEGF-ASODN tranfection reduced the VEGF mRNA and VEGF protein expression in gastric cancer cells and supernate remarkably. It also reduced survivin protein expression and increased apoptosis in gastric cancer cells.
Conclusions:Transfection with VEGF-ASODN to gastric cancer cells SGC-7901 can reduce the expression of VEGF, and can increase apoptosis and suppress the proliferation of gastric cancer cells.