Objective:To investigate the effect of combined use of antisense oligonucleotide （cmyb ASON） and vascular endothelial growth factor (VEGF) on restenosis after angioplasty.
:Methods :China rabbits were randomly divided to control group, VEGF treatment group, cmyb ASON treatment group, cmyb ASON +VEGF treatment group. Each group consisted of 7 rabbits with the same lesion of left common carotid artery. Evaluation on restenosis was done by pathological section and immunohistochemistry, and WesternBlotting for the expression of PCNA.
:Results:All groups had varying degrees of vascular endothelial and smooth muscle hyperplasia, especially in control group. The hyperplasia in cmyb ASON and VEGF groups were better than control group (P＜0.01), and cmyb ASON + VEGF combination group was baller than cmyb ASON or VEGF groups (P＜0.01). The results of WesternBlotting were similar to those of pathological section.
:Conclusions:Combined use of cmyb ASON and VEGF has definite effect in preventing restenosis after angioplasty, but cmyb ASON and VEGF do not have interrelated synergism.