Abstract:Objective:To investigate the protective effects of sphingosine-1-phosphate (S1P) on lung injury (L1) in acute necrotizing pancreatitis rats.
Methods :Thirty-six Wistar rats were allocated to sham operation group, acute necrotizing pancreatitis model group and S1P pretreatment group. Bronchoalveolar lavage fluid (BALF) and tissues from the left lung and pancreas were collected 6h after the model induction. BALF protein content, BALF leucocyte and neutrophil count, and lung tissue myeloperoxidase (MPO) activity were examined. Serum amylase and lipase were determined. In addition, histopathological changes of the pancreas and lung were observed under light microscope.
Results:BALF protein content, BALF leucocyte and neutrophil count, and lung tissue myeloperoxidase (MPO) activity were all reduced significantly in S1P pretreatment group compared to those in acute necrotizing pancreatitis model group (P<0.01).The pathological injury of the lung was obviously less severe in S1P group compared to that in model group. The serum amylase and lipase levels and the pathological damage of the pancreas were not reduced in S1P group.
Conclusions:S1P has a significant protective effect on LI in SAP rats,and it may be used as a clinical therapeutic strategy for acute necrotizing pancreatitis.