Abstract:Objective:To investigate the structural, physical performance and antigenic changes of allogenic small vessels after treatment by removal of endothelial cells, gelsiccation and irradiation. This research was intended to learn whether treated allogenic small vessels are suitable for blood vessel transplantation.
Methods :The blood vessels were randomly divided into A group and B group, in which A group was control group while B group had removal of endothelial cells as well as gelsiccation and irradiation. The tissue structure of each group was examined by light microscopy,and the condition after removal of endothelial cells was examined under electron microscope.The dilated diameter, expanded length and the largest force that the vessels could sustain in transverse and perpendicular directions when the vascular tension reached 15, 30, 45, 60, 90, 120N and 180N were calculated, and the degree of change both in transverse and perpendicular directions of the vessels under such forces was calcalated. Finally, the expressive level of major histocompatibility complex (MHC)-Ⅱantigens both pre-and post treatment was detected using immunohistochemistry S-P statistic way.
Results:The blood vessels maintained almost the same tissue structure after treatment in B group, and the changed rates both in transverse and perpendicular directions of the vessels under different tensions of the two groups had no statistical significance (P＞0.05). However, in B group, the greatest tension that the vessels could withstand in the transverse and perpendiculaar direction at the time of rupture was significantly greater than that of the control(P<0.01 and P<0.05, respectively). The MHC-Ⅱantigen level of B group was significantly lower compared to control group (P＜0.01).
Conclusions:There is no obvious change in tissue structure of allogenic blood vessels after treatment by removal of endothelial cells, gelsiccation and irradiation, meanwhile, the blood vessels could maintain certain physical characteristics. The MHC-Ⅱantigen level decreased dramatically. The vessel after treated with the method of B group basically meets the demands for vascular transplantation, and is an ideal material for such kind of operation.