Abstract:Objective:To explore the effect of amino-functional Fe3O4 nanoparticles-mediated pcDNA3.1(+)-p53(-p53) on human hepatoma cell line-HepG2 cell.
Methods :(1) Use of functional Fe3O4 nanoparticle as a vector to transfect-p53 into HepG2 cells, calulate the transfected rate, and compare it with lipofectamine vector group, blank functional Fe3O4 nanoparticles(FFN) group and control group. (2) Observation of the trasfected rate and velocity of FFN under addition of extra-magnetic field. (3) Observation of the inhibition effect of trasfected-p53 on HepG2 cells growth.
Results:(1) RT-PCR and Western blot examination comfirmed that FFN could successfully transfect-p53 into HepG2 cells, the transfection rate(39%) was slightly higher than that of liposome (36%)(P>0.05). (2) The transfection rate and velocity could be increased by addition of extra-magnetic field. (3) Transfected-p53 obviously inhibited the growth of HepG2 at G1 phase; the growth inhibition rate of HepG2 was 46%.
Conclusions:(1) FFN is a good vector for gene transfection, it can successfully transfect the gene into target cells. (2) Transefected-p53 can effectively inhibit the growth of HepG2. This result can establish a foundation for animal experimental research of gene therapy of malignancy.