Abstract:Objective:To establish a novel canine model of severe acute cholangitis (ACST).
Methods :Ten dogs were randomly divided into two groups: model group and control group. ACST models were established by placing T-tube in common bile duct and ligating the distal bile duct, then injecting E.coli trough T-tube and closing the T-tube. The changes of peripheral WBC, total bilirubin, ALT, AKP, IL-1β, TNF-α and endotoxin were observed pre-operatively and at 24 h, 48 h, 72 h, and 7 d post-operatively. The pathological changes of bile duct were observed when the dogs were sacrificed.
Results:There were no significant differences in all indices between two groups pre-operatively. Serum IL-1β,TNF-α, endotoxin and total bilirubin, ALT, AKP level in model group increased gradually and were higher than control group (P<0.05) post-operatively. Marked acute inflammation of bile duct was observed in model group.
Conclusions:This method of establishing canine ACST model can be used in related studies of ACST.