Abstract:Objective:To study the effect of NF-κB signaling pathway for HBx on up-regulation of alpha-fetoprotein (AFP).
Methods :On the basis of the establishment of LO2-HBx cell line with stable transfected HBx gene, NF-κB signaling pathway blocker PDTC was introduced to cut off its signal transduction, double fluorescent staining and laser scanning confocal microscopy were applied to study the activation and deactivation of NF-κB signaling pathway, and real-time PCR and Western Blot were facilitated to observe the expression of AFP gene before and after the HBx transfection as well as treating with PDTC.
Results:The NF-κB signaling pathway of Lo2-HBx cells was activated after transfection with HBx gene. When compared with control Lo2 cells without treatment, the mRNA and protein levels of AFP in Lo2-HBx cells increased (2.78±0.43) and (4.72±0.53) times respectively, this difference was of statistical significance（P<0.05）. But the mRNA levels of AFP decreased to (1.40±0.16), and at the same time the expression of AFP also reduced to (3.12±0.44)（P<0.05） when the NF-κB signaling pathway was blocked after treated by 50μmol/L PDTC for 24h respectively.
Conclusions:NF-κB signaling pathway is one of the routes for HBx to up-regulate the expression of AFP.