Abstract:Objective:To observe the effect of cold preservation and warm ischemia on apoptosis of hepatocytes in rats.
Methods :The hepatocyte apoptosis rate and regenerative rate of cold preservation group and warm ischemia group were detected and compared through flow cytometry.
Results:There was no difference in the hepatocyte apoptosis rate and regenerative rate(P＜0.05) at different time points during cold preservation. The hepatocyte apoptosis rate increased as the warm ischemia with time incresed (P＜0.05); whereas there was no difference in regenerative rate(P>0.05).
Conclusions:The hepatocyte apoptosis of rats is more severe after aerobic reperfusion during cold preservation /reperfusion. It is more severe after ischemia and reperfusion during warm ischemia/reperfusion periods.