Abstract:Objective:To establish a model of pancreatic cancer (PC) in SD rats,and to study the changs of serum levels of AMS and TNF-α and the significances.
Methods :Dimethylbenzanthracene (DMBA) was directly implanted into pancreatic parenchyma of SD rats (experimental group,group A), and in the process of establishing PC, weekly TSA by 1P was done in intervention group(group B). The tumor development of rats executed within 3～5 months in Group A and Group B were observed by HE staining and gross examination. Meanwhile, the rats in the sham operation group (Group C) were executed at 5 months. The levels of serum AMS were detected by autobiochemical assay apparatus, and the levels of serurn TNF-α were determined by ELISA.
Results:(1)The incidence of pancreatic cancer in Group A within 3～5 months was 48.7%(18/37),including 17 cases of pancreatic ductal adenocarcinoma and 1 case of fibrosarcoma.The incidence of pancreatic cancer in Group B was 33.3%(12/36), including 11 pancreatic ductal adenocarcinoma and 1 case of fibrosarcoma.The maximal diameter of tumor mass in Group A was higher than that in Group B(（P＜0.05）.No pathological changes were found in pancreas of Group C and in other main organs of Group A and Group B.(2) The serum levels of AMS and TNF-α were singnificantly higher in Group A and Group B than those in Group C（P＜0.05 or P＜0.01）.The serum levels of AMS and TNF-α were significantly higher in pancreatic cancer rats and non-cancerous rats of Group A and Group B than those in Group C （P＜0.05）.
Conclusions:A large dose of DMBA directly implanted into the parenchyma of pancreas can induce an ideal pancreatic cancer model with high incidence of occurrence in a short time.TSA may have an inhibitive effect on the carcinogenesis and growth of pancreatic cancer in rats. The serum levels of AMS and TNF-α are significantly increased in the process of carcinogenesis, and TNF-α might have an effect on carcinogenesis of pancreatic cancer in rats.