Abstract:Objective:To investigate the relationship between inducible nitric oxide synthase (iNOS ) mRNA expression of pancreatic tissues and intestinal permeability, and the effect of salviae miltiorrhizae on the change in gut permeability in rats with severe acute pancreatitis(SAP).
Methods :All rats were randomly divided into three groups: Sham operation（group C, n=15），SAP group（group SAP, n=15），and salviae miltiorrhizae therapeutic group （group T, n=15）. At 24h After operation, the iNOS mRNA expressions of pancreas were determined by in situ hybridization (ISH), and blood amylase(AMY), nitric oxide (NO) and lipopolysaccharide(LPS) were examined. The histopathologic changes of pancreas and ileum were observed. Intestinal permeability was measured by means of albumin clearance( AC)of 125I-labeled rat serum albumin.
Results:The iNOS mRNA expressions of pancreas in group SAP were markedly increased in comparison with group T (P＜0.01), while there was no iNOS mRNA expression of pancreas in group C. The blood AML, NO, LPS and ascites and intestinal permeability in group SAP were much higher than those of group C(P＜0.01),and also higher than those of group T (P＜0.01) except AC. The pathological changes of pancreas and ileum in group SAP were more severe than in group T(P＜0.01), however pathology of pancreas and ileum was normal in group C.
Conclusions:Excess NO produced by pancreatic iNOS mRNA overexpression may be a factor related to intestinal permeability in SAP. Salvia miltiorrhiza can down-regulate the iNOS mRNA expression of pancreas and improve the intestinal permeability in SAP.