Abstract:Objective:To establish the standard method to extract total protein of pancreatic tissue by using different methods of pancreatic tissue protein extraction in rats.
Methods :The total protein extract kit and the author self-made lysate were used to extract the total protein of pancreatic tissue, then the protein level was measured and the protein was analysed by matrix -assisted laser desorption ionization-time of flight-mass spectra( MALDI-TOF-MS) respectively.
Results:The amount of total protein extracted with total protein extract kit and self-made lysate was （19.5±1.3 ）mg and （22.2±1.8 ）mg respectively. The MALDI-TOF-MS protein spectra peak was (404.4±13.7) and( 437.2±18.1) respectively.
Conclusions:The self-made lysate extraction method has higher protein extraction rate and better protein dissolubility as compared with total protein extract kit extraction method.