Abstract:Objective: To investigate the change of the invasive and metastasis ability of HepG2 cells by silencing BC047440 genes.
Methods :The experiment were divided in 3 groups: in terfering group, HepG2/HK group and HepG2 group.Small interfering RNAs for BC047440 gene were initially designed and silenced to BC047440 genes, then the cell adhesion was measured with MTT assay, the cell migration was surveyed by scratch method, and the cell invasion in vitro was determined with Boyden chamber.
Results:There was no significant difference of the adhesion rate in the interfering group compared with HepG2/HK group and HepG2 group at 20 min and 40 min after seeding, while there was significant difference at 60min (P＜0.05). The scratch wound had almost been filled with HepG2/HK group and HepG2 group at 48h after scratching, while it was still apparent in the interfering group. The number of cells that penetrated polycarbonate coated with matrigel when incubation for 16h in interfering group was much less than HepG2/HK group and HepG2 group (P＜0.05).
Conclusions:The silencing BC047440 genes could significantly inhibit the ability of adhesion to extracellular matrix, the ability of migration to scratch wound, the ability of invasion of HepG2 cells in vitro, and these results suggest that BC047440 could regulate the invasive and metastasis ability of HepG2 cells.