Objective:To investigate the influence of tumor necrosis factor-α inhibitor on kidney injury in rats with severe acute pancreatitis (SAP).Methods:60 SD rats were randomly divided into 3 groups(n=20 in each group),including sham group, SAP group, and TNF-α Ⅰ treatment group. After the models established, the rats were killed after 48h. The levels of serum amylase, tumor necrosis factor-α, urea nitrogen and creatinine were detected. Apoptotic cells were detected by TUNEL.Nuclear-κB was detected by immunohistochemical method. The expression ofET-1 mRNA was detected by RT-PCR.Results:All of the indicators in sham group were significantly lower than those in treatment group and SAP group (P＜0.05). The levels of tumor necrosis factor-α,serum amylase, urea nitrogen, creatinine and nuclear-κB IOD were （185.36±10.95）ng/L,（7 257.30±361.20）U/L,（17.28±0.87）mmol/L,（78.83±3.02）μmol/L, 316.25±20.90 in the SAP group and （124.32±15.11）ng/L,（6182.60±291.63）U/L,（13.66±0.88）mmol/L,（68.68±3.38）μmol/L,241.90±19.04 in treatment group,the difference was statistical between the 2 groups (P＜0.05), while the apoptotic index in the SAP group (3.33±0.49) was markedly lower than that in the treatment group(7.04±0.41) (P＜0.05). The expression of ET-1 mRNA in the SAP group was higher than that in the treatment group and sham proup (P＜0.05).Conclusions:The tumor necrosis factor-α inhibitor can promote the cell apoptosis of kidney, ameliorate the kidney injury caused by inflammation, and improve kidney function.