Objective:To explore the changes of plasma endotoxin and D-lactate during intestinal mucosal barrier injury of acute pancreatitis.Methods:Wistar rats were randomly divided into sham-operation(SO)group,and severe acute pancreatitis (SAP)group. Plasma endotoxin and D-lactate were determined at 1,3,6 and 12 hour after operations.Results:The plasma endotoxin and D-lactate in SAP group increased obviously with time,and the difference betueen the 2 groups was significante(P<0.05).Endotoxin and D-lactate were positively correlated.Conclusions:The plasms endotoxin and D-lactate are increased during gut injury of SAP,and can be used as indexes to evaluate the function of the intestinal mucosal barrier.