Objective:To study the differentially expressed genes in the vascular tissue of diabetic gangrene toot and the possible mechanism of diabetic gangrene.Methods:The differentially expressed genes in the vascular tissue of diabetic gangrene foots were screened by the functional classific gene chip.Reverse transcription polymerase chain reaction (RT-PCR) detection was used to verify the differentially expressed 3 genes.
Results:In the detected 113 genes, 13 of the gene expression level increased more than 2 times, 3 of the gene expression level decreased more than 2 times. Three genes were verified with RT-PCR detection,which were results similar to those with chip testing.Conclusions:The abnormal expression in a variety of genes in tissues of vascular lesions plays an important role in the course of development and progression of diabetic gangrene. Analysis of differential gene expression can contribute to understanding of the possible mechanisms of diabetic gangrene.