Objective:To study the anatomic features of radical lymphadenectomy in laparoscopic surgery for gastric cancer and find a proper approach for laparoscopic lymph nodes resection.Methods:Via 29 cases of laparoscopic radical resection of distal gastric cancer and 6 cadaver dissections,we obsered the anatomic mark point in laparoscopic vision, available anatomic space and the differences from open-operation.Results:Twenty-fire patients underwent laparoscopic radical gastrectomy, 4 patients had conversion to open surgery for tumor invasion or fixed lymph nodes. The number of harvested lymph nodes was 11~32.The potential space between mensentery and ligament. pancreas,middle colic vessel and left gastric vessel can be used as mark points for laparoscopic surgery for distal gastric cancer,due to their obvious and fixed site. Conclusions:Lymphadenectomy in the right space by use of anatomic mark point in laparoscopic vision is safer and more standardized.Laparoscopic anatomic features can provide theoretical basis for laparoscopic surgery for gastric cancer.