Experimental study of Kupffer cell transfected with a recombinant adenoviral vector expressing interleukin 12 on proliferation of LoVo human colorectal cancer cells
Objective:To explore the effect of Kupffer Cells (KC) transfected with a recombinant adenoviral vector expressing interleukin 12 (IL-12) on human colorectal cancer LoVo cells. Methods:ELISA method was used to detect quantitatively the level of IL-12 of Kupffer cells transfected with Adv-mIL-12. KC,KC-adv-EGFP and KC-Adr-mlL-12 with LoVo cells were cultured in different plates. The cells viability and the supernatants′ VEGF were measured by MTT assay and VEGF ELISA kit；different supernatants groups of LoVo cells were harvested and differences of apoptosis were analyzed using FCM. Results:A high level of secretion of IL-12 was detected in the adv-mIL-12-transfected KC［(564.29±31.24)pg/mL 24 h］. The MTT assay and ELISA test showed that the inhibition ratio of LoVo cells was higher in the experimental group compared with 2 control groups（P＜0.05）；the FCM showed that more apoptosis cells were detected in the the experimental group(21%). Conclusions:KC-Adv-mIL-12 can inhibit the proliferation and metastasis of LoVo cells.