Objective:To investigate the malignant biologic behavioral significance of Pirh2mRNA, Her-2,VEGF,MVD and TNF-alpha in stage I and II breast cancer.
Methods :Randomly selected 87 stage I or stage II (according to AJCC, 2003) pathological samples of breast cancer, which were operated and were followed-up by the Department of Mammary Gland of Xiangya Hospital from Jan. 1996 to Oct. 2005. Hybridization in situ was used to detect the expression of Pirh2mRNA, and immunohistochemical SABC method to detect Her-2,VEGF,MVD and TNF-alpha expression in those tissue samples. Combining the clinical pathological parameters, the multifactorial Cox proportional hazards model was founded to analyze the influence of Pirh2mRNA on prognosis of breast cancer.
Results:All patients were female, aged 26 to 71 years, with the median age of 44 years. Distant metastasis within 5 years after operation occurred in 40 cases(Group A),and disease free survival for 5 years was found in 47 eases (Group B).The expression of Pirh2mRNA in group A (57.5%) was higher significantly than that of group B（21.3%）（P=0.0009）.There was no correlation between Pirh2mRNA and Her-2 (r=0.020,P＝0.856). The Kaplan-Meier survival analysis revealed the prognosis of the patients with positive expression of Pirh2mRNA,Her-2,VEGF,MVD and TNF-alpha was worse than the patients with negative results(all P<0.05). Multifactorial Cox proportional hazards model indicated that Pirh2mRNA,Her-2,VEGF,MVD and TNF-alpha were independent prognotic factors of stage I and II breast cancer.
Conclusions:The positive expression of Pirh2mRNA,Her-2,VEGF and TNF-α imply unfavourable prognosis of stage I and II breast cancer.