Objective:To observe the expression of MTA1 and ER in breast cancer, the correlation of the two factors and with the invasive capability of breast cancer.
Methods :The expression of MTA1 and ER in normal breast, precancerous lesions and breast cancer tissues was detected by using nucleic acid hybridization in situ(ISH) and immunocytochemistry(IHC) methods, and their correlation was analyzed by Spearman method.
Results:The expression of MTA1 and ER was higher in ISH than in IHC. The mRNA expression of MTA1 in normal breast tissue, precancerous lesions and breast cancer tissne was 12.2%，33.3%, and 81.1% respectively, and the expression by IHC was 11.1%, 31.1% and 72.2% respectively. The mRNA expression of ER in normal breast tissue, precancerous lesions and breast cancer tissue was 83.3%，61.1% and 37.8%,respectively, and the expression by IHC was70.9%，56.7% and 35.6% respectively.The positive expression of MTA1 was higher in ER-negative patients than that in ER-positive ones(86.2﹪vs.46.9﹪).
Conclusions:Combined ISH and IHC detection can improve the detection rate of MTA1 and ER. With advancement of the disease and lowering of tumor differentiation,the expression of MTA1 gradually increases,while expression of ER decreases and even disappears.The expression of MTA1 is negative in relation to that of ER (the coefficient is -0.466).MTA1and ER could be important molecular markers for the prognosis and therapy of breast cancer.