To investigate the alteration of lymphocyte P-gp expression level and function in liver transplant recipient and its effect on immunosuppressive therapy.
Methods :Using flow cytometry, the level of lymphocyte P-gp and analyzed the P-gp function in 53 cases of liver transplant recipieints was observed.
Results:Among 53 cases, the preoperative mean level of lymphocyte P-gp was (10.34±4.0)%, P-gp level began to increase in the 1st month postoperatively（17.8±8.0)%, peaked in the 3rd month（27.5±13.3)%， and then was stable at this level thereafter.There were no significantly difference between different sex of age group.But the P-gp level in CD+4T cells was significantly higher than that in CD+8T cells and B cells,RH123test showed that high P-gp expression of lymphocyte enhanced its transport activity of medical agents,and decreased the intracellular accumulation of drug more significantly than that of low P-gp expression (P＜0.05).
Conclusions:The P-gp expression of lymphocyte is overexpressed in some liver transplant recipients, and transport activity of P-gp was increased. There is a possibility that high P-gp expression of lymphocyte in the liver trasplant recipients may attenuate the therapeutic efficacy of immunosuppressants.