To study the protective effect of deferoxamine preconditioning on transplanted liver and its possible mechanism.
Methods :SD rats are randomly divided into four groups: sham operation group(S), autologous liver transplantation group(AT), deferoxamine preconditioning AT group(DP) and deferoxamine irrigation AT group(DI).At 24 hours after operation, several factors of each group were measured respectively, including deterioration of histomorphology, serum hepatic enzymes,and hypoxia inducing factor-1（HIF-1α）and malonaldehyde（MDA）in hepatic tissue.
Results:The levels of alanine aminotransferase(ALT), glutamic oxalacetic transaminase(AST) and alkaline phosphatase (ALP) in AT group, DP group and DI group were higher than those of S group.In the DP and DI groups, abnormal changes in liver morphology were milder,the level of HIF-1α in hepatic tissue was increased and MDA content was decreased (P﹤0.05),as compared to the AT group.
Conclusions:Deferoxamone preconditioning of graft before liver transplantion or deferoxanine inrigation of liver during operation has protective effect on donor liver, and protective effect of the former method is better than the latter.The mechanism of this preconditioning may be related to increacing HIF-1α and reducing lipid peroxidation in hepatic tissue.