Objective:To investigate the protective effect of Calcitonin Gene-related Peptide (CGRP) on ET-1 mediated injury of human hepatocyte.
Methods :Human liver tissues obtained from patients undergoing partial hepatectomies were randomly divided into five groups:control group, liver perfused with D-Hank′s solution group;liver perfused with ET-1 group and three liver perfased with ET plus CGRP (10-6M,10-7M,10-8M) treated groups. Collagenase digestion method was used to isolate human hepatocytes, then hepatocytes were cultured, and the level of MDA and TNF-α, the viability and proliferation of hepatocyte, and the hepatocyte function (ALT, Alb, Urea and LDH) were determined.
Results:As compared with control group, in ET-1 group, the viability and proliferation of hepatocytes, the level of Alb and Urea declined significantly (P<0.05), and the concentration of ALT, LDH, MDA and TNF-α increased markedly (P<0.05). In CGRP groups, the viability and proliferation of hepatocytes, the level of Alb and Urea increased significantly (P<0.05), the concentration of ALT and LDH, the level of TNF-α and MDA markedly decreased with concentration(all P<0.05).
Conclusions:CGRP has protective effect on injury of hepatocytes induced by ET-1,which may be through decrease the release of MDA and TNF-α.