Objective:To study the expression of cyclooxygenase2 in hepatic inflammatory reaction of rats with sepsis and its effect on metabolism.
Methods :Fifty-four Wistar rats were randomly divided into 3 groups: Sham (A) group, sepsis model (B) group,and NS398 intervention (C) group.The rats were subjected to cecal ligation and puncture(CLP) for sepsis model or sham operation.RT-PCR was used to determine COX-2 mRNA expression,serum prealbumin (PA) and transferrin (TF) by radioimmunity.At the same time, ALT,AST,albumin determination and liver pathological changes were determined.
Results:(1)The expression of COX-2 mRNA was low in A group,higher in B and C groups,and higher in B than C group at each time period(P＜0.05); (2)Serum ALT and AST were markedly increased in B than in A and C groups(P＜0.05); (3)Pathologic ally, hepatic injury was severe in B group, but mild in C group; (4)The levels of PA,IF and Ab were higher in A group than in B and C groups，but was lower in B group than C group（P＜0.05）.
Conclusions:COX-2 plays an important role of hepatic injury in sepsis,and can promote catabolism and inhibit anabolism. The selective cox-2 inhibitor,NS398, can effectively protect hepatic cell in sepsis and attenuate albuminolysis.